Immune activities such as growth promotion and NK cell growth promotion were compared with conventional extracts. In the present invention, after extracting ephedra using ultra-high pressure energy, the useful materials were separated and purified through sequential fractionation. EH and GF treatment suppressed the increased body temperature. HPLC analysis {(A): 3 standard, (B): ephedra fraction according to the present invention},  Example 4 Structural Analysis of Useful Bioactive Components. Our studies further support the notion that 1-phenylpropane-1,2-dione and (S)-cathinone are biosynthetic precursors of the ephedrine alkaloids in E. sinica stems and that the activity of (S)-cathinone reductases directs and determines the stereochemical branching of the pathway. Overall, the number of severe liver injury cases was significantly higher from supplements than conventional medications (P=0.02). doi: 10.1016/j.jep.2009.07.040. Network pharmacology suggests biochemical rationale for treating COVID-19 symptoms with a Traditional Chinese Medicine.  [Best form for implementation of the invention]. Anti-arthritic effects of Ephedra sinica STAPF herb-acupuncture: inhibition of lipopolysaccharide-induced inflammation and adjuvant-induced polyarthritis. Solvent 99% C0 2) Extraction temperature 30-40 ° C, pressure. Analysis of the peaks revealed that the mass and structure of the unknown isolate was ephedrine. <28> The present invention is to increase the elution of the ephedra useful bioactive substance ephedrine through the extraction of ephedra, and the reaction of H 2 S0 4 and ethyl alcohol directly with ephedra extract in the first fractionation conditions after the fractionation when the existing ephedrine extraction It is characterized by simplifying the hassle of three consecutive extractions of diethyl ether. Inform. The present invention relates to a method for isolating ephedrine from Ephedra sinica Stapf, which is a representative medicinal herb, at a high yield, and to an adjuvant for enhancing immunity containing the ephedrine as an active ingredient. Data sources include IBM Watson Micromedex (updated 2 Nov 2020), Cerner Multum™ (updated 2 Nov 2020), ASHP (updated 23 Oct 2020) and others. 1933, will also be found in China. 3, 12: 11. The optimum conditions for yield improvement in the separation of ephedrine from ephedra were presented, and the extraction amount was experimentally separated and compared using TLC / HPLC analysis. 2 mm, shallowly furrowed. Caractéristiques générales de la plante, des feuilles et des fleurs. The use of ephedra-containing products in sports has been reported.Tokish 2004, Avois 2006, Dhar 2005 Few trials evaluating the ergogenic efficacy of ephedrine alone exist, and results suggest slight effects on performance.Tokish 2004, Shekelle 2003 However, combinations of ephedrine and caffeine have been reported to increase endurance in running and cycling experiments.Dhar 2005, Shekelle 2003 Most studies have been conducted by one groupBell 2001, Bell 2002 and because of the different types of exercise studied (endurance and power), the results cannot be pooled for analysis.Shekelle 2003 Because most classes of amphetamines are banned by the International Olympic Committee (except for medical use of ephedrine) and ephedra-containing supplements are banned by the FDA, further trials evaluating their efficacy are unlikely.Tokish 2004, Avois 2006.  <78> Using Bruker Avance DPX 600 Mhz spectrometer (Germany) for structural analysis of the TLC spot of ephedra fraction obtained by the process according to the present invention — NMR (Magnetic resonance imaging) nuclear magnetic resonance (NMR) , Varian UI 500 MHz) spectra were obtained and the peaks were resolved and integrated using Win NMR software. Zhang DH, Zhang X, Peng B, Deng SQ, Wang YF, Yang L, Zhang KZ, Ling CQ, Wu KL. Epub 2006 Jan 11. -, Shaw D. Toxicological risks of Chinese herbs. The root of E. sinica or E. intermedia, known as "ma huang gen," is considered by Chinese traditional practitioners to be a distinct drug product from the aboveground parts. Until now, the useful components derived from natural products have been complicated to isolate the isolated material, and the low immune activity compared to the complex process of obtaining a useful material has been a problem. After re-extracted with diethyl ether to obtain ephedrine in a supercritical process. Ma huang is one of the earliest and best known drugs of Chinese traditional medicine. Inflammatory response in the hypothalamus by thermal stressors may affect thermal and energy homeostasis. Treatments in Other Floras @ www.efloras.org Select one or more newsletters to continue. GC–MS analysis of freshly picked young E. sinica stems enabled the detection of 1-phenylpropane-1,2-dione and (S)-cathinone, the first two putative committed biosynthetic precursors to the ephedrine alkaloids. Irritating and sweet ethyl ethers are widely used as solvents for bromine, iodine, most fats and resins, gasoline, pure rubber, and vegetable alkaloids. TLC analysis (1: ephedra fraction obtained by the existing process, 2-1: ephedra fraction obtained by the process except ultra-high pressure treatment in the process of the present invention, 2-2: ephedra fraction obtained by the process of the present invention, * : Ephedrine spot of ephedra fraction obtained by the process of the present invention),  Example 3 Analysis of Separation Yield of Ephedrine by Prep-HPLC.  <99> The toxicity of HEK293 cells of the isolated material by the process of the present invention,   Fig. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. A chapter on ephedra has been included in the Flora of China, a collaborative plant project.Lee 2011, USDA 2007, Chaw 2000, Bowe 2000, Fu 2000.

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